Anti-CD80 Antibody (6N172) is an antibody targeting CD80. Anti-CD80 Antibody (6N172) can be used in ELISA,IHC,IP,WB.

Rabbit IgG

6N172

Human

1. Western Blot
-Positive WB detected in: Raji whole cell lysate
-All lanes: CD80 antibody at 1:2000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 34, 30, 19 kDa
-Observed band size: 60 kDa
2. IHC image of TMAH-00223 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
3. Immunoprecipitating CD80 in Raji whole cell lysate
-Lane 1: Rabbit control IgG instead of TMAH-00223 in Raji whole cell lysate.
For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
-Lane 2: TMAH-00223(2µg)+ Raji whole cell lysate(500µg)
-Lane 3: Raji whole cell lysate (10µg)

WB:1:500-1:5000; IHC:1:50-1:200; IP:1:200-1:1000.

Monoclonal

Unconjugated

A synthetic peptide: Human CD80

Human

CD80

Immunology, Stem cells