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Anti-CD80 Antibody (6N172)
还可以产品编号 TMAH-00223
别名 LAB7, CD80 molecule, CD80, CD28LG1, BB1, B7-1, B71, B7.1, B7
Anti-CD80 Antibody (6N172) 是一种抗体，靶向 CD80。Anti-CD80 Antibody (6N172) 可用于 ELISA, WB, IHC, IP。
Anti-CD80 Antibody (6N172)
Anti-CD80 Antibody (6N172)
还可以产品编号 TMAH-00223 别名 LAB7, CD80 molecule, CD80, CD28LG1, BB1, B7-1, B71, B7.1, B7
Anti-CD80 Antibody (6N172) 是一种抗体，靶向 CD80。Anti-CD80 Antibody (6N172) 可用于 ELISA, WB, IHC, IP。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,300 | 5日内发货 | |
| 100 μL | ¥ 2,195 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-CD80 Antibody (6N172) is an antibody targeting CD80. Anti-CD80 Antibody (6N172) can be used in ELISA, WB, IHC, IP. |
| 别名 | LAB7, CD80 molecule, CD80, CD28LG1, BB1, B7-1, B71, B7.1, B7 |
| Ig Type | Rabbit IgG |
| 克隆号 | 6N172 |
| 交叉反应 | Human |
| 验证活性 | 1. Western Blot -Positive WB detected in: Raji whole cell lysate -All lanes: CD80 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 34, 30, 19 kDa -Observed band size: 60 kDa 2. IHC image of TMAH-00223 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 3. Immunoprecipitating CD80 in Raji whole cell lysate -Lane 1: Rabbit control IgG instead of TMAH-00223 in Raji whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) -Lane 2: TMAH-00223(2µg)+ Raji whole cell lysate(500µg) -Lane 3: Raji whole cell lysate (10µg) |
| 应用 | ELISA, WB, IHC, IP |
| 推荐剂量 | WB:1:500-1:5000; IHC:1:50-1:200; IP:1:200-1:1000. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Membrane; Single-pass type I membrane protein. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| 研究背景 | Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. (Microbial infection) Acts as a receptor for adenovirus subgroup B. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide: Human CD80 |
| 抗原种属 | Human |
| 基因ID | 941 |
| Uniprot ID | |
| 研究领域 | Immunology, Stem cells |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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