Anti-CD8 alpha Antibody (1R433) is an antibody targeting CD8 alpha. Anti-CD8 alpha Antibody (1R433) can be used in ELISA, WB, IHC, IP.

Rabbit IgG

1R433

Human

1. Western Blot
-Positive WB detected in: MCF-7 whole cell lysate, THP-1 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate
-All lanes: CD8A antibody at 1:2000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 26, 22, 31 kDa
-Observed band size: 31 kDa
2. IHC image of TMAH-00229 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
3. IHC image of TMAH-00229 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
4. Immunoprecipitating CD8A in HepG2 whole cell lysate
-Lane 1: Rabbit control IgG instead of TMAH-00229 in HepG2 whole cell lysate.
For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
-Lane 2:TMAH-00229(3µg)+ HepG2 whole cell lysate(500µg)
-Lane 3: HepG2 whole cell lysate (10µg)

ELISA, WB, IHC, IP

WB:1:500-1:5000; IHC:1:50-1:200; IP:1:200-1:1000.

Monoclonal

Unconjugated

A synthetic peptide: Human CD8

Human

925

Immunology