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Anti-CD7 Antibody (4Z579) 是一种 Rabbit 抗体,靶向 CD7。Anti-CD7 Antibody (4Z579) 可用于 WB,IP。
Anti-CD7 Antibody (4Z579) 是一种 Rabbit 抗体,靶向 CD7。Anti-CD7 Antibody (4Z579) 可用于 WB,IP。
产品描述 | Anti-CD7 Antibody (4Z579) is a Rabbit antibody targeting CD7. Anti-CD7 Antibody (4Z579) can be used in WB,IP. |
Ig Type | Monoclonal Rabbit IgG |
克隆号 | 4Z579 |
交叉反应 | Human |
验证活性 | 1. Anti-CD7 rabbit monoclonal antibody at 1:500 dilution. -Lane A: Jurkat Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:25 kDa. -Observed band size:37 kDa. 2. CD7 was immunoprecipitated using: -Lane A:0.5 mg Jurkat Whole Cell Lysate. -2 µL anti-CD7 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-CD7 rabbit monoclonal antibody, at 1:200 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 34 kDa. -Observed band size: 34 kDa |
应用 | WB,IP |
推荐剂量 | WB: 1:500-1:2000; IP: 4-6 μL/mg of lysate |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human CD7 (rh CD7; TMPY-01195; NP_006128.1; Met1-Pro180). |
纯化方式 | Protein A |
性状 | Liquid |
缓冲液 | 0.2 μm filtered solution in PBS |
研究背景 | The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD7 is a transmembrane protein that is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell / B-cell interaction during early lymphoid development. |
偶联 | Unconjugated |
免疫原 | Recombinant Human CD7 protein (TMPY-01195) |
抗原种属 | Human |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
运输方式 | Shipping with blue ice. |
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