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Anti-CD53 Antibody (6D899)
还可以产品编号 TMAY-03431
Anti-CD53 Antibody (6D899) 是一种 Rabbit 抗体，靶向 CD53。Anti-CD53 Antibody (6D899) 可用于 WB,IP。
Anti-CD53 Antibody (6D899)
Anti-CD53 Antibody (6D899)
还可以产品编号 TMAY-03431
Anti-CD53 Antibody (6D899) 是一种 Rabbit 抗体，靶向 CD53。Anti-CD53 Antibody (6D899) 可用于 WB,IP。
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抗原信息
| 产品描述 | Anti-CD53 Antibody (6D899) is a Rabbit antibody targeting CD53. Anti-CD53 Antibody (6D899) can be used in WB,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 6D899 |
| 交叉反应 | Mouse |
| 验证活性 | 1. Anti-CD53 rabbit monoclonal antibody at 1:500 dilution. -Lane A: Raji Whole Cell Lysate. -Lane B: Jurkat Whole Cell Lysate. -Lane C: 293T Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:24 kDa. -Observed band size:15 kDa. 2. Mouse CD53 was immunoprecipitated using: -Lane A:0.5 mg 293T Whole Cell Lysate. -Lane B:0.5 mg Raji Whole Cell Lysate. -2 µL anti-Mouse CD53 rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein G. -Primary antibody: -Anti-Mouse CD53 rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 24 kDa. -Observed band size: 24 kDa |
| 应用 | WB,IP |
| 推荐剂量 | WB: 1:500-1:2000; IP: 0.5-2 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD53 (rM CD53; TMPY-03939; Q61451; Glu107-Asn181). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | CD53 is a member of the transmembrane 4 superfamily, also called the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. These proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD53 is a cell surface glycoprotein that is known to complex with integrins. Familial deficiency of CD53 gene has been linked to an immunodeficiency associated with recurrent infectious diseases caused by bacteria, fungi and viruses. CD53 contributes to the transduction of CD2-generated signals in T cells and natural killer cells and has been suggested to play a role in growth regulation. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Mouse CD53 Protein (TMPY-03939) |
| 抗原种属 | Mouse |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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