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Anti-CD5 Antibody (1B432)

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货号 TMAB-04017

Anti-CD5 Antibody (1B432) 是一种 Rabbit 抗体，靶向 CD5。Anti-CD5 Antibody (1B432) 可用于 IHC-Fr,IHC-P,IF,WB。

Anti-CD5 Antibody (1B432)

Anti-CD5 Antibody (1B432)

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Rating icon 还可以
货号 TMAB-04017

Anti-CD5 Antibody (1B432) 是一种 Rabbit 抗体，靶向 CD5。Anti-CD5 Antibody (1B432) 可用于 IHC-Fr,IHC-P,IF,WB。

规格价格库存数量
25 μL
¥ 795
5日内发货
50 μL
¥ 1,395
5日内发货
100 μL
¥ 2,795
5日内发货
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产品介绍

生物活性
产品描述
Anti-CD5 Antibody (1B432) is a Rabbit antibody targeting CD5. Anti-CD5 Antibody (1B432) can be used in IHC-Fr,IHC-P,IF,WB.
克隆号
1B432
反应种属
Human
验证活性
1. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD5 Monoclonal Antibody, Unconjugated (TMAB-04017) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD5 Monoclonal Antibody, Unconjugated (TMAB-04017) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD5 Monoclonal Antibody, Unconjugated (TMAB-04017) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD5 Monoclonal Antibody, Unconjugated (TMAB-04017) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
5. 25 μg total protein per lane of various lysates (see on figure) probed with CD5 monoclonal antibody, unconjugated (TMAB-04017) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
6. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD5 Monoclonal Antibody, Unconjugated (TMAB-04017) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nuclei.
7. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with CD5 Monoclonal Antibody, Unconjugated (TMAB-04017) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nuclei.
应用IHC-FrIHC-PIFWB
推荐剂量
IHC-Fr: 1:50-200; IHC-P: 1:50-200; IF: 1:50-200; WB: 1:500-2000
抗体种类
Monoclonal
宿主来源Rabbit
亚细胞定位Cell membrane; Single-pass type I membrane protein.
构建方式Recombinant Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1mg/ml
研究背景This gene encodes a member of the scavenger receptor cysteine-rich (SRCR) superfamily. Members of this family are secreted or membrane-anchored proteins mainly found in cells associated with the immune system. This protein is a type-I transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes and a subset of B lymphocytes. The encoded protein contains three SRCR domains and may act as a receptor to regulate T-cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2016]
抗原信息
免疫原
A synthesized peptide: human CD5
抗原种属
Human
基因名称
CD5
基因ID
921
蛋白名称
T-cell surface glycoprotein CD5
Uniprot ID
P06127
功能
May act as a receptor in regulating T-cell proliferation.
化学信息
分子量Theoretical: 55 kDa. Actual: 67 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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