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Anti-CD48 Antibody (1M803) 是一种 Rabbit 抗体,靶向 CD48。Anti-CD48 Antibody (1M803) 可用于 ELISA(Det),FCM,ICC/IF。
Anti-CD48 Antibody (1M803) 是一种 Rabbit 抗体,靶向 CD48。Anti-CD48 Antibody (1M803) 可用于 ELISA(Det),FCM,ICC/IF。
产品描述 | Anti-CD48 Antibody (1M803) is a Rabbit antibody targeting CD48. Anti-CD48 Antibody (1M803) can be used in ELISA(Det),FCM,ICC/IF. |
Ig Type | Monoclonal Rabbit IgG |
克隆号 | 1M803 |
交叉反应 | Mouse |
验证活性 | 1. Immunofluorescence staining of Mouse CD48 in Mouse Spleen cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse CD48 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cell membrane. 2. Flow cytometric analysis of Mouse CD48 expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse CD48, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
应用 | ELISA(Det),FCM,ICC/IF |
推荐剂量 | ICC-IF: 1:20-1:100; FCM: 1:25-1:100; ELISA(Det): 1:1000-1:10000 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD48 / SLAMF2 (rM CD48 / SLAMF2; TMPY-01372; NP_031675.1; Met1-Arg216). |
纯化方式 | Protein A |
性状 | Liquid |
缓冲液 | 0.2 μm filtered solution in PBS |
研究背景 | Cluster of Differentiation 48 (CD48), also known as SLAMF2, BCM-1 and BLAST-1, is a GPI-linked protein belonging to the CD2 subfamily of immunoglobulin superfamily molecules. CD2 and 2B4 (CD244) are known ligands for CD48. CD48 protein is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors. CD48 protein performs biological functions in a variety processes including adhesion, pathogen recognition, cellular activation, and cytokine regulation, and emerges as a critical effector molecule in immune responses.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: ICC AntibodiesImmune Checkpoint Detection: IHC AntibodiesImmune Checkpoint Detection: IP AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint ProteinsImmune Checkpoint TargetsImmunotherapyTargeted Therapy |
偶联 | Unconjugated |
其他可选修饰符 | APC/PerCP/FITC/HRP/PE |
可提供抗体种类 | 6 |
免疫原 | Recombinant Mouse CD48 / SLAMF2 protein (TMPY-01372) |
抗原种属 | Mouse |
研究领域 | Cancer Drug Targets |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
运输方式 | Shipping with blue ice. |
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