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Anti-CD40 Polyclonal Antibody
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产品编号 TMAB-00365
Anti-CD40 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 CD40。Anti-CD40 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-CD40 Polyclonal Antibody
Anti-CD40 Polyclonal Antibody
还可以产品编号 TMAB-00365
Anti-CD40 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 CD40。Anti-CD40 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,170 | 5日内发货 | |
| 100 μL | ¥ 1,975 | 5日内发货 | |
| 200 μL | ¥ 2,795 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-CD40 Polyclonal Antibody is a Rabbit antibody targeting CD40. Anti-CD40 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat,Rabbit (predicted:Chicken,Dog,Pig,Cow) |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (human tonsil tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD40) Polyclonal Antibody, Unconjugated (TMAB-00365) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Lane 1: Spleen (Mouse) Lysate at 40 μg Lane 2: Lung (Mouse) Lysate at 40 μg Lane 3: LympHnode (Rat) Lysate at 40 μg Primary: Anti-CD40/TNFRSF5 (TMAB-00365) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44-50 kDa Observed band size: 50 kDa 3. Sample: Hela (Human) Cell Lysate at 30 μg Primary: Anti-CD40 (TMAB-00365) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kDa Observed band size: 52 kDa 4. Sample: Jurkat (Human) Cell Lysate at 30 μg Raw264.7 (Mouse) Cell Lysate at 30 μg Primary: Anti-CD40 (TMAB-00365) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kDa Observed band size: 52 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Isoform I: Cell membrane; Single-pass type I membrane protein. Isoform II: Secreted. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | Predicted to enable antigen binding activity; protein domain specific binding activity; and ubiquitin protein ligase binding activity. Involved in B cell mediated immunity; CD40 signaling pathway; and cellular calcium ion homeostasis. Acts upstream of or within several processes, including defense response to other organism; positive regulation of B cell activation; and positive regulation of interleukin-12 production. Located in external side of plasma membrane and intracellular membrane-bounded organelle. Part of CD40 receptor complex. Is expressed in several structures, including alimentary system; brain; hemolymphoid system gland; liver and biliary system; and reproductive system. Human ortholog(s) of this gene implicated in several diseases, including Kawasaki disease; autoimmune disease (multiple); end stage renal disease; hyperimmunoglobulin syndrome (multiple); and non-Hodgkin lymphoma (multiple). Orthologous to human CD40 (CD40 molecule). [provided by Alliance of Genome Resources, Apr 2022] |
| 免疫原 | KLH conjugated synthetic peptide: human CD40/TNFRSF5. |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Innate and adaptive immunity,CD,B Lymphocytic Lineage,Dendritic Cell Lineage |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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