Anti-CD4 Antibody (2J310)

Name: Anti-CD4 Antibody (2J310)
Brand: TargetMol
SKU: TMAY-03133
Rating: 4.5 (1 reviews)

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产品编号 TMAY-03133

Anti-CD4 Antibody (2J310) 是一种 Rabbit 抗体，靶向 CD4。Anti-CD4 Antibody (2J310) 可用于 FCM。

Anti-CD4 Antibody (2J310)

还可以

产品编号 TMAY-03133

Anti-CD4 Antibody (2J310) 是一种 Rabbit 抗体，靶向 CD4。Anti-CD4 Antibody (2J310) 可用于 FCM。

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生物活性

产品描述	Anti-CD4 Antibody (2J310) is a Rabbit antibody targeting CD4. Anti-CD4 Antibody (2J310) can be used in FCM.
Ig Type	Monoclonal Rabbit IgG
克隆号	2J310
交叉反应	Mouse
验证活性	Flow cytometric analysis of Mouse CD4 expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse CD4, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
应用	FCM
推荐剂量	FCM: 1:25-1:100
抗体种类	Monoclonal
宿主来源	Rabbit
构建方式	This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD4 (rM CD4; TMPY-01226; NP_038516.1; Met1-Thr394).
纯化方式	Protein A
性状	Liquid
缓冲液	0.2 μm filtered solution in PBS
研究背景	T-cell surface glycoprotein CD4, is a single-pass type I membrane protein. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. The CD4 surface determinant, previously associated as a phenotypic marker for helper/inducer subsets of T lymphocytes, has now been critically identified as the binding/entry protein for human immunodeficiency viruses (HIV). The human CD4 molecule is readily detectable on monocytes, T lymphocytes, and brain tissues. All human tissue sources of CD4 bind radiolabeled gp120 to the same relative degree; however, the murine homologous protein, L3T4, does not bind the HIV envelope protein. CD4 is a co-receptor that assists the T cell receptor (TCR) to activate its T cell following an interaction with an antigen-presenting cell. Using its portion that resides inside the T cell, CD4 amplifies the signal generated by the TCR. CD4 interacts directly with MHC class II molecules on the surface of the antigen-presenting cell via its extracellular domain. The CD4 molecule is currently the object of intense interest and investigation both because of its role in normal T-cell function, and because of its role in HIV infection. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors.Viral protein U (VpU) of HIV-1 plays an important role in downregulation of the main HIV-1 receptor CD4 from the surface of infected cells. Physical binding of VpU to newly synthesized CD4 in the endoplasmic reticulum is an early step in a pathway leading to proteasomal degradation of CD4. Amino acids in both helices found in the cytoplasmic region of VpU in membrane-mimicking detergent micelles experience chemical shift perturbations upon binding to CD4, whereas amino acids between the two helices and at the C-terminus of VpU show no or only small changes, respectively. Paramagnetic spin labels were attached at three sequence positions of a CD4 peptide comprising the transmembrane and cytosolic domains of the receptor. VpU binds to a membrane-proximal region in the cytoplasmic domain of CD4.

偶联与修饰

偶联	Unconjugated
其他可选修饰符	APC/FITC/PE
可提供抗体种类	4

抗原信息

免疫原	Recombinant Mouse CD4 Protein (TMPY-01226)
抗原种属	Mouse

存储&运输

储存方式	Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式	Shipping with blue ice.