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Anti-CD34 Polyclonal Antibody
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产品编号 TMAB-00357
Anti-CD34 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 CD34。Anti-CD34 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF,IP。
Anti-CD34 Polyclonal Antibody
Anti-CD34 Polyclonal Antibody
还可以产品编号 TMAB-00357
Anti-CD34 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 CD34。Anti-CD34 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF,IP。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,170 | 5日内发货 | |
| 100 μL | ¥ 1,960 | 5日内发货 | |
| 200 μL | ¥ 2,785 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-CD34 Polyclonal Antibody is a Rabbit antibody targeting CD34. Anti-CD34 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,IP. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (TMAB-00357) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus. 2. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (TMAB-00357) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus. 3. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (TMAB-00357) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus. 4. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (TMAB-00357) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus. 5. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (TMAB-00357) at 1:100 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus. 6. 25 μg total protein per Lane of various lysates probed with CD34 polyclonal antibody, unconjugated (TMAB-00357) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 7. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (TMAB-00357) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (TMAB-00357) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (TMAB-00357) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 10. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (TMAB-00357) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 11. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (TMAB-00357) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 12. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (TMAB-00357) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 13. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (TMAB-00357) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 14. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (TMAB-00357) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 15. Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (TMAB-00357) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 16. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CD34 Polyclonal Antibody, Unconjugated (TMAB-00357) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; IP: 1:20-50 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Membrane; Single-pass type I membrane protein. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence. |
| 免疫原 | KLH conjugated synthetic peptide: human CD34 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Tumor biomarkers,Endothelial Cell Markers,Stem cells,Mesoderm,Myeloid Cells,Soma marker,Endothelial Markers,HSC markers,Surface Molecules,Mesoderm,Negative Markers |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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