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Anti-CD34 Antibody (1K692)
还可以产品编号 TMAY-00259
Anti-CD34 Antibody (1K692) 是一种 Mouse 抗体，靶向 CD34。Anti-CD34 Antibody (1K692) 可用于 WB,IP。
Anti-CD34 Antibody (1K692)
Anti-CD34 Antibody (1K692)
还可以产品编号 TMAY-00259
Anti-CD34 Antibody (1K692) 是一种 Mouse 抗体，靶向 CD34。Anti-CD34 Antibody (1K692) 可用于 WB,IP。
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偶联与修饰
抗原信息
| 产品描述 | Anti-CD34 Antibody (1K692) is a Mouse antibody targeting CD34. Anti-CD34 Antibody (1K692) can be used in WB,IP. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 1K692 |
| 交叉反应 | Human |
| 验证活性 | 1. Anti-CD34 mouse monoclonal antibody at 1:500 dilution. -Lane A: TF-1 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:41 kDa. 2. pCMV3-CD34-TT-WPRE was immunoprecipitated using: -Lane A:0.5 mg TF-1 Whole Cell Lysate. -4 µL anti-pCMV3-CD34-TT-WPRE mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G. -Primary antibody: -Anti-pCMV3-CD34-TT-WPRE mouse monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size: 41 kDa. -Observed band size:95 kDa |
| 应用 | WB,IP |
| 推荐剂量 | WB: 1:500-1:2000; IP: 1-5 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with full length human CD34 expression plasmid. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Cluster of Differentiation 34 (CD34) is a member of a family of single-pass transmembrane sialomucin proteins, and may function as a cell-cell adhesion factor. CD34 protein is selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. It has been widely used as a stem and progenitor cell marker, and clinical CD34+ stem cell transplantation (CD34+SCT) has been performed for tumor purging. CD34 monoclonal antibodies are widely used to identify and isolate hemopoietic progenitors and to classify acute and chronic leukemias. |
| 偶联 | Unconjugated |
| 免疫原 | Full length human CD34 expression plasmid |
| 抗原种属 | Human |
| 研究领域 | Cardiac Stem Cell Markers, Hemangioblast Markers |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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