Anti-CD31/PECAM-1 Antibody (9Y633) is an antibody targeting CD31/PECAM-1. Anti-CD31/PECAM-1 Antibody (9Y633) can be used in ELISA, WB, FCM.

Mouse IgG2a

9Y633

Human, Mouse

1. Western Blot
-Positive WB detected in: THP-1 whole cell lysate, JK whole cell lysate
-All lanes: PECAM1 antibody at 1:500
-Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution
-Predicted band size: 82-140 kDa
-Observed band size: 140 kDa
2. Overlay Peak curve showing THP-1 cells stained with TMAH-00875 (red line) at 1:100. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, WB, FCM

WB:1:1000-1:5000; FCM:1:20-1:200.

Monoclonal

Unconjugated

Recombinant Protein: Human PECAM1 Protein

Human

5175

Cancer, Cardiovascular, Immunology, Signal transduction, Stem cells