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Anti-CD3 epsilon/CD3e Antibody (2H914)

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Anti-CD3 epsilon/CD3e Antibody (2H914)
产品编号 TMAY-01399
别名 CD3e molecule, ε (CD3-TCR complex), CD3e molecule, epsilon (CD3-TCR complex), CD3 ε/CD3e

Anti-CD3 epsilon/CD3e Antibody (2H914) 是一种 Rabbit 抗体，靶向 CD3 epsilon/CD3e。Anti-CD3 epsilon/CD3e Antibody (2H914) 可用于 WB,IP。

Anti-CD3 epsilon/CD3e Antibody (2H914)
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Anti-CD3 epsilon/CD3e Antibody (2H914)

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Anti-CD3 epsilon/CD3e Antibody (2H914)
产品编号 TMAY-01399 别名 CD3e molecule, ε (CD3-TCR complex), CD3e molecule, epsilon (CD3-TCR complex), CD3 ε/CD3e

Anti-CD3 epsilon/CD3e Antibody (2H914) 是一种 Rabbit 抗体，靶向 CD3 epsilon/CD3e。Anti-CD3 epsilon/CD3e Antibody (2H914) 可用于 WB,IP。

规格价格库存数量
100 μL
¥ 2,279
5日内发货
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产品介绍

生物活性
产品描述
Anti-CD3 epsilon/CD3e Antibody (2H914) is a Rabbit antibody targeting CD3 epsilon/CD3e. Anti-CD3 epsilon/CD3e Antibody (2H914) can be used in WB,IP.
别名CD3e molecule, ε (CD3-TCR complex), CD3e molecule, epsilon (CD3-TCR complex), CD3 ε/CD3e
Ig Type
Rabbit IgG
克隆号
2H914
交叉反应
Human
验证活性
1. CD3E was immunoprecipitated using:
-Lane A:0.5 mg Jurkat Whole Cell Lysate.
-Lane B:0.5 mg HuT78 Whole Cell Lysate.
-2 µL anti-CD3E rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-CD3E rabbit monoclonal antibody, at 1:100 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 23 kDa.
-Observed band size: 23 kDa.
2. Anti-CD3E rabbit monoclonal antibody at 1:500 dilution.
-Lane A: Hu-T78 Whole Cell Lysate.
-Lane B: Jurakt Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:23 kDa.
-Observed band size:23 kDa(We are unsure as to the identity of these extra bands.)
应用
WB,IP
推荐剂量
WB: 1:500-1:2000; IP: 1-4 μL/mg of lysate
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Human CD3e / CD3 epsilon (rh CD3e / CD3 epsilon; TMPY-01191; NP_000724.1; Met1-Asp126).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 epsilon subunit of the T cell receptor (TCR) complex contains two defined signaling domains, a proline-rich sequence and an immune tyrosine activation motifs (ITAMs), and this complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells. In the CD3 epsilon mutant mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated, including activation of mature naïve T cells with the MHCII presented superantigen, staphylococcal enterotoxin B, or with a strong TCR cross-linking antibody specific for either TCR-Cbeta or CD3 epsilon. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. Homozygous mutations in CD3D and CD3E genes lead to a complete block in T-cell development and thus to an early-onset severe combined immunodeficiency phenotype.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: Human CD3e / CD3 epsilon Protein (TMPY-01191)
抗原种属
Human
研究领域
ITIM/ITAM Immunoreceptors and Related Molecules
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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关键词

CD3-TCR complexCD3e molecule, epsilon

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