Anti-CD2 Antibody (7A731) is a Rabbit antibody targeting CD2. Anti-CD2 Antibody (7A731) can be used in WB,ICC/IF,FCM.

IgG

7A731

Human

1. 25 μg total protein per lane of various lysates (see on figure) probed with CD2 monoclonal antibody, unconjugated (TMAB-03904) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. 4% Paraformaldehyde-fixed Molt-4 (H) cell; Antibody incubation with (CD2) monoclonal Antibody, unconjugated (TMAB-03904) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
3. The Molt-4 (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Rabbit Anti-CD2 antibody (TMAB-03904): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

WB: 1:500-2000; ICC/IF: 1:50-200; FCM: 1μg/Test

Monoclonal

A synthesized peptide: human CD2

Human

CD2

T-cell surface antigen CD2

CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.