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Anti-CD14 Antibody (7E908)

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Anti-CD14 Antibody (7E908)
产品编号 TMAC-00658
别名 monocyte differentiation antigen CD14, CD14 molecule, CD14 antigen

Anti-CD14 Antibody (7E908) 是一种 Rabbit 抗体，靶向 CD14。Anti-CD14 Antibody (7E908) 可用于 WB,ICC,IHC。

Anti-CD14 Antibody (7E908)

Anti-CD14 Antibody (7E908)

Rating icon 还可以
Anti-CD14 Antibody (7E908)
产品编号 TMAC-00658 别名 monocyte differentiation antigen CD14, CD14 molecule, CD14 antigen

Anti-CD14 Antibody (7E908) 是一种 Rabbit 抗体，靶向 CD14。Anti-CD14 Antibody (7E908) 可用于 WB,ICC,IHC。

规格价格库存数量
50 μL
¥ 1,495
5日内发货
100 μL
¥ 2,495
5日内发货
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产品介绍

生物活性
产品描述
Anti-CD14 Antibody (7E908) is a Rabbit antibody targeting CD14. Anti-CD14 Antibody (7E908) can be used in WB,ICC,IHC.
别名monocyte differentiation antigen CD14, CD14 molecule, CD14 antigen
Ig Type
IgG
交叉反应
Human
验证活性
1. Western blot analysis of CD14 on different lysates using anti-CD14 antibody at 1/1,000 dilution. Positive control: Lane 1: Human liver, Lane 2: SW480.
2. Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-CD14 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD14 antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CD14 antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CD14 antibody. Counter stained with hematoxylin.
6. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD14 antibody. Counter stained with hematoxylin.
7. ICC staining CD14 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. ICC staining CD14 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
9. ICC staining CD14 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
10. ICC staining CD14 in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
应用
WB
ICC
IHC
推荐剂量
WB: 1:1000-2000; IHC: 1:50-200; ICC: 1:50-200
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景Lipopolysaccharide (LPS) elicits the secretion of mediators and cytokines produced by activated macrophages and monocytes. CD14 is a glycosylphosphatidylinositol (GPI)-anchored protein found on the surfaces of monocytes and polymorphonuclear leukocytes. CD14 functions as a receptor for LPS, resulting in the secretion of various proteins. An important component in the LPS activation of monocytes through the CD14 receptor is the "adapter molecule," lipopolysaccharide binding protein (LBP). There are two forms of CD14, a membrane-associated form (mCD14), and a soluble form (sCD14). mCD14 responds to LPS alone and facilitates the secretion of proteins, while cells not expressing mCD14 fail to respond to LPS. The cells that lack mCD14 respond to LPS/LBP in the presence of sCD14.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
P08571
化学信息
分子量Theoretical: 55-65 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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