Anti-CD11a Polyclonal Antibody 2 is a Rabbit antibody targeting CD11a. Anti-CD11a Polyclonal Antibody 2 can be used in FCM.

IgG

Human (predicted:Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep)

Blank control: U937 (blue).
Primary Antibody: Rabbit Anti-Integrin alpha L antibody (TMAB-03822), Dilution: 0.2 μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions);
Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1: 200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (TMAB-03822, 0.2 μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA+1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

FCM: 0.2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human Integrin alpha L

Human

ITGAL

Integrin alpha-L

Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.