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Anti-CD10 Antibody (5I446)
还可以产品编号 TMAY-01182
Anti-CD10 Antibody (5I446) 是一种 Mouse 抗体，靶向 CD10。Anti-CD10 Antibody (5I446) 可用于 WB,IHC-P,IP。
Anti-CD10 Antibody (5I446)
Anti-CD10 Antibody (5I446)
还可以产品编号 TMAY-01182
Anti-CD10 Antibody (5I446) 是一种 Mouse 抗体，靶向 CD10。Anti-CD10 Antibody (5I446) 可用于 WB,IHC-P,IP。
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偶联与修饰
抗原信息
| 产品描述 | Anti-CD10 Antibody (5I446) is a Mouse antibody targeting CD10. Anti-CD10 Antibody (5I446) can be used in WB,IHC-P,IP. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 5I446 |
| 交叉反应 | Human |
| 验证活性 | 1. Anti-MME mouse monoclonal antibody at 1:500 dilution. -Lane A: Raji Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:85 kDa. 2. Immunochemical staining of human CD10 in human lymphoma with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections. 3. Immunochemical staining of human CD10 in human Submandibular gland inflammation with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections. 4. Immunochemical staining of human CD10 in human uterus cancer with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections. 5. MME was immunoprecipitated using: -Lane A:0.5 mg Raji Whole Cell Lysate. -4 µL anti-MME mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G. -Primary antibody: -Anti-MME mouse monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size: 85 kDa. -Observed band size:100 kDa |
| 应用 | WB,IHC-P,IP |
| 推荐剂量 | WB: 1:500-1:2000; IHC-P: 1:20-1:100; IP: 1-5 µL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD10 (rh CD10; TMPY-02315; NP_000893.2; Tyr52-Trp750). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alters the behavior of the cell. Some CD proteins do not take part in the cell signal process but have other functions such as cell adhesion. The cluster of differentiation 10 (CD10), also known as Neprilysin and neutral endopeptidase, is a member of the CD system. CD10 is a zinc-dependent metalloprotease enzyme that had the function to degrade some small secreted peptides such as the amyloid beta-peptide. It exists as a membrane-bound protein and has a high concentration in kidney and lung tissues. Mutations in the CD10 gene can induce the familial forms of Alzheimer's disease, providing strong evidence for the protein's association with the Alzheimer's disease process. CD10 is also associated with other biochemical processes. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human CD10 Protein (TMPY-02315) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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