Anti-CCL25 Polyclonal Antibody is a Rabbit antibody targeting CCL25. Anti-CCL25 Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P.

IgG

Rat (predicted:Human,Mouse,Dog,Pig,Cow,Horse,Rabbit)

1. Paraformaldehyde-fixed, paraffin embedded (rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TECK) Polyclonal Antibody, Unconjugated (TMAB-00308) at 1:400 overnight at 4°C, followed by a conjugated secondary at [1:500] for 90 minutes and DAPI staining of the nucleus.
2. Paraformaldehyde-fixed, paraffin embedded (rat thymus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TECK) Polyclonal Antibody, Unconjugated (TMAB-00308) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat thymus); Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TECK) Polyclonal Antibody, Unconjugated (TMAB-00308) at 1:400 overnight at 4°C, followed by a conjugated secondary at [1:500] for 90 minutes and DAPI staining of the nucleus.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human CCL25

Human

CCL25

C-C motif chemokine 25

Beta Chemokines (CC)

Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1.