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Anti-CCDC11 Polyclonal Antibody

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货号 TMAB-03730

Anti-CCDC11 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 CCDC11。Anti-CCDC11 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-CCDC11 Polyclonal Antibody

Anti-CCDC11 Polyclonal Antibody

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Rating icon 还可以
货号 TMAB-03730

Anti-CCDC11 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 CCDC11。Anti-CCDC11 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,160
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100 μL
¥ 1,975
5日内发货
200 μL
¥ 2,780
5日内发货
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产品介绍

生物活性
产品描述
Anti-CCDC11 Polyclonal Antibody is a Rabbit antibody targeting CCDC11. Anti-CCDC11 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
反应种属
Human,Mouse,Rat (predicted:Cow,Horse)
验证活性
1. Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CCDC11) Polyclonal Antibody, Unconjugated (TMAB-03730) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Sample: A431 (Human) Cell Lysate at 30 μg
Primary: Anti-CCDC11 (TMAB-03730) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 63 kD
3. 25 μg total protein per lane of various lysates (see on figure) probed with CCDC11 polyclonal antibody, unconjugated (TMAB-03730) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
应用WBIHC-PIHC-FrIF
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1mg/ml
研究背景CCDC11 is a 514 amino acid protein encode by a gene that maps to human chromosome 18q21.1. Encoding over 300 genes, chromosome 18 contains about 76 million bases. Trisomy 18, or Edwards syndrome, is the second most common trisomy after Downs syndrome. Symptoms of Edwards syndrome include low birth weight, a variety of physical development defects, heart deformations and breathing difficulty. Translocation between chromosome 18 and 14 is the most common translocation in cancers, and occurs in follicular lymphomas. Niemann-Pick disease, hereditary hemorrhagic telangiectasia and erythropoietic protoporphyria are associated with chromosome 18. The TGF∫ modulators, Smad2, Smad4 and Smad7 are encoded by chromosome 18.
抗原信息
免疫原
KLH conjugated synthetic peptide: human CCDC11
抗原种属
Human
基因名称
CFAP53
基因ID
220136
蛋白名称
Cilia-and flagella-associated protein 53
Uniprot ID
Q96M91
化学信息
分子量Theoretical: 57 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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