Anti-CAV1 Polyclonal Antibody is a Rabbit antibody targeting CAV1. Anti-CAV1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit,Sheep)

1. Paraformaldehyde-fixed, paraffin embedded (Rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Caveolin-1) Polyclonal Antibody, Unconjugated (TMAB-00299) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 min and DAB staining.
2. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Caveolin-1 Polyclonal Antibody, Unconjugated (TMAB-00299) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Tissue/cell: mouse retina tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Caveolin-1 Polyclonal Antibody, Unconjugated (TMAB-00299) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
4. Sample:
Lung (Mouse) Lysate at 40 μg
Bladder (Mouse) Lysate at 40 μg
Primary: Anti-Caveolin-1 (TMAB-00299) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 20 kDa
5. Sample:
NIH/3T3 Cell Lysate at 40 μg
A549 Cell Lysate at 40 μg
HUVEC Cell Lysate at 40 μg
Primary: Anti-Caveolin-1 (TMAB-00299) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 20 kDa
6. Sample:
Lung (Mouse) Lysate at 40 μg
Primary: Anti-Caveolin-1 (TMAB-00299) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 20 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Caveolin-1

Human

Response to hypoxia,Tumor biomarkers,Cholesterol Metabolism,Cholesterol Metabolism,Hypoxia,Coat Proteins,Caveolae and Clathrin