Anti-Cathepsin L Polyclonal Antibody 2 is a Rabbit antibody targeting Cathepsin L. Anti-Cathepsin L Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Rat (predicted:Mouse)

1. Sample:
A549 Cell (Human) Lysate at 30 μg
K562 Cell (Human) Lysate at 30 μg
Primary: Anti-Cathepsin L (TMAB-03690) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19/30/37 kD
Observed band size: 40 kD
2. Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Cathepsin L Polyclonal Antibody, Unconjugated (TMAB-03690) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Cathepsin Polyclonal Antibody, Unconjugated (TMAB-03690) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
4. Blank control: A549.
Primary Antibody (green line): Rabbit Anti-Cathepsin L antibody (TMAB-03690)
Dilution: 1 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
5. Sample:
Lane 1: Human HUVEC cell Lysates
Lane 2: Human K562 cell Lysates
Lane 3: Human MCF-7 cell Lysates
Primary: Anti-Cathepsin L (TMAB-03690) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19/30/37 kDa
Observed band size: 40 kDa
6. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cathepsin L) Polyclonal Antibody, Unconjugated (TMAB-03690) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human cathepsin L1 proprotein

Human

CTSL

Procathepsin L

Thiol protease important for the overall degradation of proteins in lysosomes (Probable). Involved in the solubilization of cross-linked TG/thyroglobulin and in the subsequent release of thyroid hormone thyroxine (T4) by limited proteolysis of TG/thyroglobulin in the thyroid follicle lumen (PubMed:12782676).In neuroendocrine chromaffin cells secretory vesicles, catalyzes the prohormone proenkephalin processing to the active enkephalin peptide neurotransmitter (PubMed:12869695).In thymus, regulates CD4+ T cell positive selection by generating the major histocompatibility complex class II (MHCII) bound peptide ligands presented by cortical thymic epithelial cells (PubMed:12021314).Also mediates invariant chain processing in cortical thymic epithelial cells (PubMed:9545226).Major elastin-degrading enzyme at neutral pH. Accumulates as a mature and active enzyme in the extracellular space of antigen presenting cells (APCs) to regulate degradation of the extracellular matrix in the course of inflammation (PubMed:12417635).Secreted form generates endostatin from COL18A1 (PubMed:10716919).Critical for cardiac morphology and function (PubMed:11972068).Plays an important role in hair follicle morphogenesis and cycling, as well as epidermal differentiation (PubMed:12163394).Required for maximal stimulation of steroidogenesis by TIMP1 (By similarity).