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顶部生物活性储存和溶解信息计算器

Anti-Caspase-3 Antibody (6C392)

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Anti-Caspase-3 Antibody (6C392)
产品编号 TMAC-00081
别名 Yama, SCA-1, CPP32B, CPP32, apopain

Anti-Caspase-3 Antibody (6C392) 是一种 Rabbit 抗体，靶向 Caspase-3。Anti-Caspase-3 Antibody (6C392) 可用于 WB,ICC/IF,IHC。

Anti-Caspase-3 Antibody (6C392)

Anti-Caspase-3 Antibody (6C392)

Rating icon 还可以
Anti-Caspase-3 Antibody (6C392)
产品编号 TMAC-00081 别名 Yama, SCA-1, CPP32B, CPP32, apopain

Anti-Caspase-3 Antibody (6C392) 是一种 Rabbit 抗体，靶向 Caspase-3。Anti-Caspase-3 Antibody (6C392) 可用于 WB,ICC/IF,IHC。

规格价格库存数量
50 μL
¥ 1,490
5日内发货
100 μL
¥ 2,485
5日内发货
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产品介绍

生物活性
产品描述
Anti-Caspase-3 Antibody (6C392) is a Rabbit antibody targeting Caspase-3. Anti-Caspase-3 Antibody (6C392) can be used in WB,ICC/IF,IHC.
别名Yama, SCA-1, CPP32B, CPP32, apopain
Ig Type
IgG
克隆号
6C392
交叉反应
Human
验证活性
1. Western blot analysis of active Caspase-3 on different cell lysates using anti-active Caspase-3 antibody at 1/1,000 dilution. Positive control: Lane 1: Camptothecin treated Jurkat cells, Lane 2: Untreated Jurkat cells.
2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-active Caspase-3 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-active Caspase-3 antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-active Caspase-3 antibody. Counter stained with hematoxylin.
5. ICC staining active Caspase-3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining active Caspase-3 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
应用
WB
ICC/IF
IHC
推荐剂量
WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景Caspase-3, also known as apopain, SCA-1, Yama and CPP32, is an aspartate-specific cysteine protease that belongs to the ICE subfamily of caspases. Caspase-3 is expressed in cells as an inactive precursor from which the p17 and p11 subunits of the mature caspase-3 are proteolytically generated during apoptosis. The caspase-3 precursor is first cleaved at Asp175-Ser176 to produce the p11 subunit and the p20 peptide. Subsequently, the p20 peptide is cleaved at Asp28-Ser29 to generate the mature p17 subunit. The active caspase-3 enzyme is a heterodimer composed of two p17 and two p11 subunits. At the onset of apoptosis, caspase-3 proteolytically cleaves PARP at an Asp216-Gly217 bond. During the execution of the apoptotic cascade, activated caspase-3 releases SREBP from the membrane of the ER in a proteolytic reaction that is distinct from their normal sterol-dependent activation. Caspase-3 cleaves and activates SREBPs between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase-3 also cleaves and activates caspase-6, -7 and -9. The human caspase-3 gene encodes a cytoplasmic protein that is highly expressed in lung, spleen, heart, liver, kidney and cells of the immune system.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
P42574
化学信息
分子量Theoretical: 17 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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