Anti-Caspase-3 Antibody (3C501) is an antibody targeting Caspase-3. Anti-Caspase-3 Antibody (3C501) can be used in ELISA, WB, IHC.

Rabbit IgG

3C501

Human

1. Western Blot
-Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, JK whole cell lysate, MCF7 whole cell lysate, K562 whole cell lysate, A549 whole cell lysate
-All lanes: Caspase 3 antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 32 kDa
-Observed band size: 32 kDa
2. IHC image of TMAH-00143 diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.18% DAB.
3. IHC image of TMAH-00143 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.18% DAB.

ELISA, WB, IHC

WB:1:500-1:2000; IHC:1:50-1:200.

Monoclonal

Unconjugated

A synthetic peptide: Human CASP3

Human

836

Cancer, Cell biology, Metabolism