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Anti-Carboxypeptidase M Antibody (6K425)
还可以产品编号 TMAY-01778
Anti-Carboxypeptidase M Antibody (6K425) 是一种 Rabbit 抗体，靶向 Carboxypeptidase M。Anti-Carboxypeptidase M Antibody (6K425) 可用于 WB,ELISA,IP。
Anti-Carboxypeptidase M Antibody (6K425)
Anti-Carboxypeptidase M Antibody (6K425)
还可以产品编号 TMAY-01778
Anti-Carboxypeptidase M Antibody (6K425) 是一种 Rabbit 抗体，靶向 Carboxypeptidase M。Anti-Carboxypeptidase M Antibody (6K425) 可用于 WB,ELISA,IP。
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产品介绍
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偶联与修饰
抗原信息
| 产品描述 | Anti-Carboxypeptidase M Antibody (6K425) is a Rabbit antibody targeting Carboxypeptidase M. Anti-Carboxypeptidase M Antibody (6K425) can be used in WB,ELISA,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 6K425 |
| 交叉反应 | Human |
| 验证活性 | 1. Anti-CPM rabbit monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:51 kDa. -Observed band size:55 kDa(We are unsure as to the identity of these extra bands.) 2. CPM was immunoprecipitated using: -Lane A:0.5 mg HepG2 Whole Cell Lysate. -2 µL anti-CPM rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-CPM rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution. -Developed using the DAB staining technique. -Performed under reducing conditions. -Predicted band size: 55 kDa. -Observed band size: 55 kDa |
| 应用 | WB,ELISA,IP |
| 推荐剂量 | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human Carboxypeptidase M / CPM (rh Carboxypeptidase M / CPM; TMPY-01313; NP_938079.1; Met 1-His 422). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Carboxypeptidase M, also known as CPM, is a membrane-bound arginine/lysine carboxypeptidase which is a member of the carboxypeptidases family. These enzymes remove C-terminal amino acids from peptides and proteins and exert roles in the physiological processes of blood coagulation/fibrinolysis, inflammation, food digestion and pro-hormone and neuropeptide processing. Among the carboxypeptidases CPM is of particular importance because of its constitutive expression in an active form at the surface of specialized cells and tissues in the human body. CPM in the brain appears to be membrane-bound via a phosphatidylinositol glycan anchor. CPM is widely distributed in a variety of tissues and cells. The amino acid sequence of CPM indicated that the C-terminal hydrophobic region might be a signal for membrane attachment via a glycosylphosphatidylinositol (GPI) anchor. CPM is involved in peptide metabolism on both the cell surface and in extracellular fluids. CPM functions not only as a protease but also as a binding partner in cell-surface protein-protein interactions. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human Carboxypeptidase M / CPM Protein (TMPY-01313) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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