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Anti-CAMK2 alpha Polyclonal Antibody 2 是一种 Rabbit 抗体,靶向 CAMK2 alpha。Anti-CAMK2 alpha Polyclonal Antibody 2 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF。
Anti-CAMK2 alpha Polyclonal Antibody 2 是一种 Rabbit 抗体,靶向 CAMK2 alpha。Anti-CAMK2 alpha Polyclonal Antibody 2 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,160 | 5日内发货 | |
100 μL | ¥ 1,970 | 5日内发货 | |
200 μL | ¥ 2,790 | 5日内发货 |
Anti-CAMK2 alpha Polyclonal Antibody 2 相关产品
产品描述 | Anti-CAMK2 alpha Polyclonal Antibody 2 is a Rabbit antibody targeting CAMK2 alpha. Anti-CAMK2 alpha Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
别名 | KCC2A, CaMK-II subunit alpha, CaMKII alpha, CaMKII, CAMKB, CAMKA, CAMK2α, CAMK2D, CAMK2B, CAMK2A, CAMK2 α, CaMK II delta subunit, CaMK II beta subunit, CaMK II alpha subunit, CaMK 2a, CAM2, CaM kinase II subunit alpha, CaM kinase II delta subunit, CaM kinase II delta chain, CaM kinase II beta subunit, CaM kinase II beta chain, CaM kinase II alpha subunit, CaM kinase II alpha chain, Calcium/calmodulin-dependent protein kinase(CaM kinase) II alpha, Calcium/calmodulin-dependent protein kinase type IIA, Calcium/calmodulin-dependent protein kinase type II subunit alpha, Calcium/Calmodulin Dependent Protein Kinase II G, Calcium/calmodulin dependent protein kinase II alpha, Calcium calmodulin dependent protein kinase II alpha-B subunit, Calcium calmodulin dependent protein kinase II, Calcium calmodulin dependent protein kinase CaM kinase II alpha, alpha CaMKII |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Sample: Lane 1: Mouse Heart tissue lysates Lane 2: Rat Heart tissue lysates Primary: Anti-CaMK2 alpha (TMAB-00275) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 50 kDa 2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CaMK2 alpha) Polyclonal Antibody, Unconjugated (TMAB-00275) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. 25 μg total protein per Lane of various lysates probed with CaMK2 alpha polyclonal antibody, unconjugated (TMAB-00275) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 4. 4% Paraformaldehyde-fixed SH-SY5Y (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (CaMK2 alpha) polyclonal Antibody, unconjugated (TMAB-00275) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control. |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Cell junction, synapse, presynaptic cell membrane. Cell junction, synapse. Note=Postsynaptic lipid rafts. |
构建方式 | Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Eight transcript variants encoding eight distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]. |
免疫原 | KLH conjugated synthetic peptide: human CaMK2 alpha |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Calmodulin / CaMK,Hypoxia,Hypertrophy,Response to hypoxia |
分子量 | Theoretical: 54 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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