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Anti-Calreticulin Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Calreticulin。Anti-Calreticulin Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF,ELISA。
Anti-Calreticulin Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Calreticulin。Anti-Calreticulin Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF,ELISA。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,165 | 5日内发货 | |
100 μL | ¥ 1,965 | 5日内发货 | |
200 μL | ¥ 2,780 | 5日内发货 |
产品描述 | Anti-Calreticulin Polyclonal Antibody is a Rabbit antibody targeting Calreticulin. Anti-Calreticulin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA. |
Ig Type | IgG |
交叉反应 | Human |
验证活性 | 1. Sample: Lane 1: Human Jurkat cell lysates Lane 2: Human HepG2 cell lysates Lane 3: Human MCF-7 cell lysates Primary: Anti-Calreticulin (TMAB-00273) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kDa Observed band size: 52 kDa 2. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Polyclonal Antibody, Unconjugated (TMAB-00273) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Polyclonal Antibody, Unconjugated (TMAB-00273) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Polyclonal Antibody, Unconjugated (TMAB-00273) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Polyclonal Antibody, Unconjugated (TMAB-00273) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Polyclonal Antibody, Unconjugated (TMAB-00273) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Endoplasmic reticulum lumen. Cytoplasm, cytosol. Secreted, extracellular space, extracellular matrix. Cell surface. |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | Calreticulin is a highly conserved chaperone protein which resides primarily in the endoplasmic reticulum, and is involved in a variety of cellular processes, among them, cell adhesion. Additionally, it functions in protein folding quality control and calcium homeostasis. Calreticulin is also found in the nucleus, suggesting that it may have a role in transcription regulation. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin. Recurrent mutations in calreticulin have been linked to various neoplasms, including the myeloproliferative type.[provided by RefSeq, May 2020] |
免疫原 | KLH conjugated synthetic peptide: human Calreticulin |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | ER |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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