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Anti-Calbindin Antibody (5F708) 是一种 Rabbit 抗体,靶向 Calbindin。Anti-Calbindin Antibody (5F708) 可用于 WB,ICC/IF,IHC。
Anti-Calbindin Antibody (5F708) 是一种 Rabbit 抗体,靶向 Calbindin。Anti-Calbindin Antibody (5F708) 可用于 WB,ICC/IF,IHC。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,495 | 5日内发货 | |
100 μL | ¥ 2,490 | 5日内发货 |
产品描述 | Anti-Calbindin Antibody (5F708) is a Rabbit antibody targeting Calbindin. Anti-Calbindin Antibody (5F708) can be used in WB,ICC/IF,IHC. |
别名 | Vitamin D-dependent calcium-binding protein, avian-type, D-28K, Calbindin D28, Calbindin, CALB1, CAB27 |
Ig Type | IgG |
克隆号 | 5F708 |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of Calbindin on different lysates using anti-Calbindin antibody at 1/1,000 dilution. Positive control: Lane 1: Rat brain, Lane 2: Mouse kidney. 2. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Calbindin antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calbindin antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Calbindin antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Calbindin antibody. Counter stained with hematoxylin. 6. ICC staining Calbindin in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Calbindin in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
应用 | |
推荐剂量 | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | The family of EF-hand type Ca2+-binding proteins includes Calbindin D28K, Calbindin D9K, S-100 α and β, Calgranulin A (also designated MRP8), Calgranulin B (also designated MRP14), Calgranulin C and the Parvalbumin family members, including Parvalbumin α and Parvalbumin β (also designated oncomodulin). Calbindin D28K, also known as calbindin, CALB1, D-28K or vitamin D-dependent calcium-binding protein, is a 261 amino acid protein with six EF-hand domains, four of which are active calcium-binding domains. Expressed in brain, ovary, uterus, testis, pancreas, liver, kidney and intestine, Calbindin D28K acts as a calcium-buffering agent and alters the activity of the plasma membrane ATPase. In neuronal cells, Calbindin D28K modulates calcium channel activity, calcium transients and intrinsic neuronal firing activity. Also, Calbindin D28K has been implicated to play a role in apoptosis and microtubule function. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 28 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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