Anti-CA9/Carbonic Anhydrase IX Polyclonal Antibody is a Rabbit antibody targeting CA9/Carbonic Anhydrase IX. Anti-CA9/Carbonic Anhydrase IX Polyclonal Antibody can be used in ICC/IF,IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse)

1. Sample:
Stomach (Mouse) Lysate at 40 μg
Primary: Anti-Carbonate dehydratase IX (TMAB-00266) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 48 kDa
2. Sample:
Stomach (Rat) Lysate at 40 μg
Primary: Anti-Carbonate dehydratase IX (TMAB-00266) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 48/58 kDa
3. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CA9) Polyclonal Antibody, Unconjugated (TMAB-00266) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CA9) Polyclonal Antibody, Unconjugated (TMAB-00266) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
5. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (CA9) polyclonal Antibody, Unconjugated (TMAB-00266) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:25; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Carbonate dehydratase IX

Human

CA9

Carbonic anhydrase 9

Response to hypoxia,Associated Proteins,Vitamins / minerals,Hypoxia,Vitamins / Minerals

Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.