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Anti-CA9 Antibody (8W181) 是一种 Rabbit 抗体,靶向 CA9。Anti-CA9 Antibody (8W181) 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-CA9 Antibody (8W181) 是一种 Rabbit 抗体,靶向 CA9。Anti-CA9 Antibody (8W181) 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 25 μL | ¥ 790 | 5日内发货 | |
| 50 μL | ¥ 1,395 | 5日内发货 | |
| 100 μL | ¥ 2,485 | 5日内发货 |
| 产品描述 | Anti-CA9 Antibody (8W181) is a Rabbit antibody targeting CA9. Anti-CA9 Antibody (8W181) can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 克隆号 | 8W181 |
| 反应种属 | Human |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CA9 Monoclonal Antibody, Unconjugated (TMAB-03533) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CA9 Monoclonal Antibody, Unconjugated (TMAB-03533) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 3. 25 μg total protein per lane of various lysates (see on figure) probed with CA9 monoclonal antibody, unconjugated (TMAB-03533) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. |
| 应用 | WBIHC-PIHC-FrIF |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Nucleus. Nucleus, nucleolus. Cell membrane; Single-pass type I membrane protein. Cell projection, microvillus membrane; Single-pass type I membrane protein. Note=Found on the surface microvilli and in the nucleus, particularly in nucleolus. |
| 组织特异性 | Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 研究背景 | Catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions). |
| 免疫原 | A synthesized peptide: human Carbonic anhydrase 9 |
| 抗原种属 | Human |
| 基因名称 | CA9 |
| 基因ID | |
| 蛋白名称 | Carbonic anhydrase 9 |
| Uniprot ID | |
| 功能 | Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. |
| 分子量 | Theoretical: 50 kDa. Actual: 50 kDa. |
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |