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Anti-C5b-9 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 C5b-9。Anti-C5b-9 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-C5b-9 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 C5b-9。Anti-C5b-9 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,170 | 5日内发货 | |
100 μL | ¥ 1,965 | 5日内发货 | |
200 μL | ¥ 2,790 | 5日内发货 |
产品描述 | Anti-C5b-9 Polyclonal Antibody is a Rabbit antibody targeting C5b-9. Anti-C5b-9 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Ig Type | IgG |
交叉反应 | Human,Rat (predicted:Mouse) |
验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (C5b-9) Polyclonal Antibody, Unconjugated (TMAB-00264) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 2. Sample: Lane 1: A549 (Human) Cell Lysate at 30 μg Lane 2: Hela (Human) Cell Lysate at 30 μg Primary: Anti-C5b (TMAB-00264) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 72 kDa Observed band size: 72 kDa |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Secreted. |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | Activation of the complement system plays a key role in normal inflammatory response to injury but may cause substantial injury when activated inappropriately. The complement system is activated either through the classical (antibody induced) or the alternative (microbial surface, polysacharride induced) pathway, both leading to the formation of the C5b9 complex. Fluid phase binding of the multifunctional glycoprotein S protein (vitronectin) to C5b9 leads to the formation of a cytolytically inactive complex, SC5b9, which is unable to attach to cells. |
免疫原 | Purified Human SC5b-9 Complex |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Oxidative Stress,Alternative Pathway,Classical Pathway,MAC,Macrophage / Inflamm.,Oxidative stress,GPCR |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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