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Anti-Brachyury/TBXT Antibody (6H365)
还可以产品编号 TMAY-00125
别名 TFT, T, SAVA
Anti-Brachyury/TBXT Antibody (6H365) 是一种 Mouse 抗体，靶向 Brachyury/TBXT。Anti-Brachyury/TBXT Antibody (6H365) 可用于 ICC/IF。
Anti-Brachyury/TBXT Antibody (6H365)
Anti-Brachyury/TBXT Antibody (6H365)
还可以产品编号 TMAY-00125 别名 TFT, T, SAVA
Anti-Brachyury/TBXT Antibody (6H365) 是一种 Mouse 抗体，靶向 Brachyury/TBXT。Anti-Brachyury/TBXT Antibody (6H365) 可用于 ICC/IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 100 μL | ¥ 1,689 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-Brachyury/TBXT Antibody (6H365) is a Mouse antibody targeting Brachyury/TBXT. Anti-Brachyury/TBXT Antibody (6H365) can be used in ICC/IF. |
| 别名 | TFT, T, SAVA |
| Ig Type | Monoclonal Mouse IgG2b |
| 克隆号 | 6H365 |
| 交叉反应 | Human |
| 验证活性 | Immunofluorescence staining of TBXT in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-Human T monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to Nucleus. |
| 应用 | ICC/IF |
| 推荐剂量 | ICC-IF: 1:20-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the C-terminus of the Human Brachyury/TBXT. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Brachyury is encoded by the TBXT gene. TBXT plays in the Cnidaria, appears to be in defining the blastopore. Brachyury is an important factor in promoting the epithelial-mesenchymal transition (EMT). Cells that over-express brachyury have down-regulated expression of the adhesion molecule E-cadherin, which allows them to undergo EMT. This process is at least partially mediated by the transcription factors AKT and Snail. Brachyury is overexpressed in a number of tumor types. |
| 偶联 | Unconjugated |
| 免疫原 | A synthetic peptide corresponding to the C-terminus of the Human Brachyury/TBXT |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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