Anti-BNIP3 Polyclonal Antibody is a Rabbit antibody targeting BNIP3. Anti-BNIP3 Polyclonal Antibody can be used in FCM.

IgG

Human (predicted:Mouse,Rat,Chicken,Dog,Pig,Cow,Horse)

Blank control: A43
Primary Antibody (green line): Rabbit Anti-BNIP3 antibody (TMAB-00246)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

FCM: 2ug/Test

Polyclonal

KLH conjugated synthetic peptide: human BNIP3

Human

Host Virus Interaction,Cancer,Mitochondrial markers,Hypoxia,Apoptosis,Mitochondrial,Bcl2 Family,Apoptosis,Mitochondrial,Metabolism,Response to hypoxia