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Anti-BMP-4 Polyclonal Antibody
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产品编号 TMAB-00243
Anti-BMP-4 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 BMP-4。Anti-BMP-4 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-BMP-4 Polyclonal Antibody
Anti-BMP-4 Polyclonal Antibody
还可以产品编号 TMAB-00243
Anti-BMP-4 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 BMP-4。Anti-BMP-4 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,165 | 5日内发货 | |
| 100 μL | ¥ 1,960 | 5日内发货 | |
| 200 μL | ¥ 2,780 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-BMP-4 Polyclonal Antibody is a Rabbit antibody targeting BMP-4. Anti-BMP-4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Mouse,Sheep (predicted:Human,Rat) |
| 验证活性 | 1. Sample: Sheep skin (Sheep) Lysate at 40 μg Primary: Anti-BMP4 (TMAB-00243) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 13/45 kDa Observed band size: 50 kDa 2. Paraformaldehyde-fixed, paraffin embedded (mouse pancreas tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (BMP4) Polyclonal Antibody, Unconjugated (TMAB-00243) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Secreted, extracellular space, extracellular matrix. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | BMPs (bone morphogenetic proteins) belong to the TGF beta superfamily of structurally related signaling proteins. Members of this superfamily are widely represented throughout the animal kingdom and have been implicated in a variety of developmental processes. Proteins of the TGF beta superfamily are disulfide-linked dimers composed of two 12-15 kDa polypeptide chains. As implied by their name, BMPs initiate, promote and regulate bone development, growth, remodeling and repair. Smad1 translocation to the nucleus is observed after the addition of BMP4 (also designated BMP2B), suggesting that BMP4 may play a role in activation of the Smad pathway. BMP is secreted into the extracellular matrix. |
| 免疫原 | KLH conjugated synthetic peptide: human BMP4 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | TGF,TGF,Kidney development,Gut specification,Nervous system development,TGF,Secreted |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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