Anti-BMP-2 Polyclonal Antibody 2 is a Rabbit antibody targeting BMP-2. Anti-BMP-2 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,GuineaPig)

1. Tissue/cell: rat stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-BMP2 Polyclonal Antibody, Unconjugated (TMAB-00242) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
A431 (Human) Cell Lysate at 30 μg
Hela (Human) Cell Lysate at 30 μg
Primary: Anti-BMP2 (TMAB-00242) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 13/44 kDa
Observed band size: 64 kDa
3. Sample:
Stomach (Mouse) Lysate at 40 μg
Primary: Anti-BMP2 (TMAB-00242) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 13/44 kDa
Observed band size: 59 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human BMP2

Human

TGF,TGF,Bone,TGF,Extracellular,Secreted