Anti-beta I Tubulin Antibody (8S183), Loading Control is a Rabbit antibody targeting beta I Tubulin. Anti-beta I Tubulin Antibody (8S183), Loading Control can be used in WB,IHC-P,IHC-Fr,IF,ICC/IF,FCM.

IgG

8S183

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta I Tubulin Monoclonal Antibody, Unconjugated (TMAB-03117) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta I Tubulin Monoclonal Antibody, Unconjugated (TMAB-03117) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta I Tubulin Monoclonal Antibody, Unconjugated (TMAB-03117) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta I Tubulin Monoclonal Antibody, Unconjugated (TMAB-03117) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta I Tubulin Monoclonal Antibody, Unconjugated (TMAB-03117) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta I Tubulin Monoclonal Antibody, Unconjugated (TMAB-03117) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta I Tubulin Monoclonal Antibody, Unconjugated (TMAB-03117) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
8. 4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (beta I Tubulin) monoclonal Antibody, unconjugated (TMAB-03117) 1: 100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, FITC) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
9. 25 ug total protein per lane of various lysates (see on figure) probed with beta I Tubulin monoclonal antibody, unconjugated (TMAB-03117) at 1:50000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:50000-100000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ICC/IF: 1:100-500; FCM: 1μg/Test

Monoclonal

KLH conjugated synthetic peptide: human beta I Tubulin

Human

TUBB1

Tubulin beta-1 chain

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).