Anti-beta-Actin Antibody (8L335) is a Rabbit antibody targeting beta-Actin. Anti-beta-Actin Antibody (8L335) can be used in FCM, IF, IHC-Fr, IHC-P, WB.

IgG

8L335

Human,Mouse,Rat (predicted:Rabbit,Chicken,Hamster,Dog)

1. 25 ug total protein per lane of various lysates (see on figure) probed with beta-Actin monoclonal antibody, unconjugated (TMAB-03124) at 1:20000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. 25 ug total protein per lane of various lysates (see on figure) probed with beta-Actin monoclonal antibody, unconjugated (TMAB-03124) at 1: 100000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta-Actin Monoclonal Antibody, Unconjugated (TMAB-03124) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta-Actin Monoclonal Antibody, Unconjugated (TMAB-03124) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta-Actin Monoclonal Antibody, Unconjugated (TMAB-03124) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta-Actin Monoclonal Antibody, Unconjugated (TMAB-03124) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
7. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-beta-Actin antibody (TMAB-03124, 1:200)s; Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

FCM=1:100-500; IF=1:200-1000; IHC-Fr=1:200-1000; IHC-P=1:200-1000; WB=1:20000-200000

Monoclonal

Synthetic MAP peptide derived from human beta-Actin

Human

ACTB

Actin, cytoplasmic 1

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.