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Anti-beta 4B/2A/2B Tubulin Antibody (5R187) 是一种 Rabbit 抗体,靶向 beta 4B/2A/2B Tubulin。Anti-beta 4B/2A/2B Tubulin Antibody (5R187) 可用于 FCM, IF, IHC-Fr, IHC-P, IP, WB。
Anti-beta 4B/2A/2B Tubulin Antibody (5R187) 是一种 Rabbit 抗体,靶向 beta 4B/2A/2B Tubulin。Anti-beta 4B/2A/2B Tubulin Antibody (5R187) 可用于 FCM, IF, IHC-Fr, IHC-P, IP, WB。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 25 μL | ¥ 790 | 5日内发货 | |
| 50 μL | ¥ 1,395 | 5日内发货 | |
| 100 μL | ¥ 2,495 | 5日内发货 |
| 产品描述 | Anti-beta 4B/2A/2B Tubulin Antibody (5R187) is a Rabbit antibody targeting beta 4B/2A/2B Tubulin. Anti-beta 4B/2A/2B Tubulin Antibody (5R187) can be used in FCM, IF, IHC-Fr, IHC-P, IP, WB. |
| Ig Type | IgG |
| 克隆号 | 5R187 |
| 反应种属 | Human,Mouse,Rat |
| 验证活性 | 1. 25 ug total protein per lane of various lysates (see on figure) probed with beta 4B/2A/2B Tubulin monoclonal antibody, unconjugated (TMAB-03091) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 2. 25 ug total protein per lane of various lysates (see on figure) probed with beta 4B/2A/2B Tubulin monoclonal antibody, unconjugated (TMAB-03091) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 3. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta 4B/2A/2B Tubulin Monoclonal Antibody, Unconjugated (TMAB-03091) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with beta 4B/2A/2B Tubulin Monoclonal Antibody, Unconjugated (TMAB-03091) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. |
| 应用 | FCMIFIHC-FrIHC-PIPWB |
| 推荐剂量 | FCM=1:50-100; IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500; IP=1:20-50; WB=1:500-2000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 研究背景 | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. |
| 免疫原 | A synthesized peptide: human beta 4B Tubulin |
| 抗原种属 | Human |
| 基因名称 | TUBB4B |
| 基因ID | |
| 蛋白名称 | Tubulin beta-4B chain |
| Uniprot ID |
| 分子量 | Theoretical: 50 kDa. Actual: 50 kDa. |
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |