Anti-Beclin-1 Antibody (8U829) is a Mouse antibody targeting Beclin-1. Anti-Beclin-1 Antibody (8U829) can be used in IF,IHC-Fr,IHC-P.

IgG

8U829

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (ascites of TMAB-00208) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (ascites of TMAB-00208) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (ascites of TMAB-00208) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (ascites of TMAB-00208) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (ascites of TMAB-00208) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (ascites of TMAB-00208) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (ascites of TMAB-00208) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (ascites of TMAB-00208) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (ascites of TMAB-00208) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Rat Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (ascites of TMAB-00208) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Human Fundus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (ascites of TMAB-00208) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (ascites of TMAB-00208) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

KLH conjugated synthetic peptide: human Beclin 1

Human

BECN1

Beclin-1

Signal Transduction,Tumor suppressors,Autophagy,Tumor Suppressors,Autophagy and mitophagy,Host Immune Response

Plays a central role in autophagy. Required for the abcission step in cytokinesis. May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.