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Anti-BCLG Antibody (4H668)

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货号 TMAB-03068

Anti-BCLG Antibody (4H668) 是一种 Mouse 抗体，靶向 BCLG。Anti-BCLG Antibody (4H668) 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF。

Anti-BCLG Antibody (4H668)

Anti-BCLG Antibody (4H668)

一键复制产品信息
Rating icon 还可以
货号 TMAB-03068

Anti-BCLG Antibody (4H668) 是一种 Mouse 抗体，靶向 BCLG。Anti-BCLG Antibody (4H668) 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF。

规格价格库存数量
25 μL
¥ 785
5日内发货
50 μL
¥ 1,390
5日内发货
100 μL
¥ 2,490
5日内发货
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产品介绍

生物活性
产品描述
Anti-BCLG Antibody (4H668) is a Mouse antibody targeting BCLG. Anti-BCLG Antibody (4H668) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Ig Type
IgG/Kappa
克隆号
4H668
反应种属
Human,Mouse,Rat
验证活性
1. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:5000
Primary ab incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: A549, HepG2, Rat brain, Mouse pancreas
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 37 kDa
Observed MW: 52 kDa
2. Tissue: Human colon cancer
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-03068
3. Tissue: Mouse stomach
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-03068
4. Tissue: Rat lung
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-03068
5. Cell line: A549
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-03068
应用WBIHC-PIHC-FrICC/IFIF
推荐剂量
WB: 1:1000-5000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500
抗体种类
Monoclonal
宿主来源Mouse
亚细胞定位Cytoplasm. Isoform 1: Cytoplasm, cytosol. Note=Diffusely distributed throughout the cytosol. Isoform 2: Endomembrane system. Note=Predominantly localized to cytosolic organelles.
组织特异性Isoform 1 is widely expressed. Isoform 2 is testis-specific.
构建方式Recombinant Antibody
纯化方式Protein G purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1mg/ml
研究背景Bcl-G is a novel pro-apoptotic member of the Bcl-2 family. The human Bcl-G gene encodes two proteins through alternative mRNA splicing, Bcl-G(L) (long) and Bcl-G(S) (short) consisting of 327 and 252 amino acids in length, respectively. Bcl-G(L) mRNA is expressed widely in adult human tissues, whereas Bcl-G(S) mRNA was found only in testis. Overexpression of Bcl-G(L) or Bcl-G(S) in cells induced apoptosis although Bcl-G(S) was far more potent than Bcl-G(L).
抗原信息
基因名称
BCL2L14
基因ID
79370
蛋白名称
Apoptosis facilitator Bcl-2-like protein 14
功能
Plays a role in apoptosis.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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