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Anti-BAMBI Polyclonal Antibody

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Anti-BAMBI Polyclonal Antibody
产品编号 TMAB-00184

Anti-BAMBI Polyclonal Antibody 是一种 Rabbit 抗体,靶向 BAMBI。Anti-BAMBI Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-BAMBI Polyclonal Antibody

Anti-BAMBI Polyclonal Antibody

Rating icon 还可以
Anti-BAMBI Polyclonal Antibody
产品编号 TMAB-00184

Anti-BAMBI Polyclonal Antibody 是一种 Rabbit 抗体,靶向 BAMBI。Anti-BAMBI Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,165
5日内发货
100 μL
¥ 1,960
5日内发货
200 μL
¥ 2,780
5日内发货
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产品介绍

生物活性
产品描述
Anti-BAMBI Polyclonal Antibody is a Rabbit antibody targeting BAMBI. Anti-BAMBI Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
交叉反应
Human,Mouse (predicted:Rat,Dog,Pig,Cow,Rabbit,Sheep,GuineaPig)
验证活性
1. Sample:
Testis (Mouse) Lysate at 40 μg
Spleen (Mouse) Lysate at 40 μg
Primary: Anti-BAMBI (TMAB-00184) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kDa
Observed band size: 30 kDa
2. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-BAMBI Polyclonal Antibody, Unconjugated (TMAB-00184) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample:
Lane 1: K562 (Human) Cell Lysate at 30 μg
Lane 2: MCF-7 (Human) Cell Lysate at 30 μg
Lane 3: HepG2 (Human) Cell Lysate at 30 μg
Lane 4: A549 (Human) Cell Lysate at 30 μg
Primary:
Anti-BAMBI (TMAB-00184) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 30 kDa
4. Sample:
RAW264.7 (Mouse) CellLysate at 30 μg
Primary: Anti-BAMBI (TMAB-00184) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kDa
Observed band size: 30 kDa
应用
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Membrane.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景BAMBI is a membrane spanning glycoprotein that acts as a negative regulator of TGF-β signaling during development. The BAMBI family of proteins are related to type I TGF-β receptor family, however, BAMBI is a pseudoreceptor that lacks an intracellular serine/threonine kinase domain. BAMBI transcription regulation is under the influence of β-catenin, BMP, smad3 and smad4. BAMBI expression can increase in colorectal and hepatocellular carcinomas relative to non-cancerous tissues. BAMBI is expressed at high levels during odontogenesis. BAMBI is coexpressed with Bmp-4 during early Xenopus embryogenesis and can be detected in poorly metastatic human melanoma cell lines.
抗原信息
免疫原
KLH conjugated synthetic peptide: human BAMBI
抗原种属
Human
基因ID
Uniprot ID
研究领域
TGF,Surface Molecules
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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