Anti-Bak Antibody (3J180) is a Rabbit antibody targeting Bak. Anti-Bak Antibody (3J180) can be used in FCM, ICC/IF, IF, IHC-Fr, IHC-P, IP, WB.

IgG

3J180

Human

1. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bak Monoclonal Antibody, Unconjugated (TMAB-03021) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bak Monoclonal Antibody, Unconjugated (TMAB-03021) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Pancreatic Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Bak Monoclonal Antibody, Unconjugated (TMAB-03021) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
4. 4% Paraformaldehyde-fixed THP-1 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Bak) monoclonal Antibody, unconjugated (TMAB-03021) 1: 100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
5. The THP-1 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-Bak antibody (TMAB-03021, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
6. 25 ug total protein per lane of various lysates (see on figure) probed with Bak monoclonal antibody, unconjugated (TMAB-03021) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

FCM=1 μg/Test; ICC/IF=1:100-500; IF=1:200-500; IHC-Fr=1:200-500; IHC-P=1:200-500; IP=1:20-50; WB=1:500-2000

Monoclonal

A synthesized peptide: human BAK1

Human

BAK1

Bcl-2 homologous antagonist/killer

In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti-apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis.