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Anti-BAG3 Antibody (8D844) 是一种抗体,靶向 BAG3。Anti-BAG3 Antibody (8D844) 可用于 ELISA, WB, IHC。
Anti-BAG3 Antibody (8D844) 是一种抗体,靶向 BAG3。Anti-BAG3 Antibody (8D844) 可用于 ELISA, WB, IHC。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,310 | 5日内发货 | |
100 μL | ¥ 2,185 | 5日内发货 |
产品描述 | Anti-BAG3 Antibody (8D844) is an antibody targeting BAG3. Anti-BAG3 Antibody (8D844) can be used in ELISA, WB, IHC. |
别名 | MFM6, Docking protein CAIR-1, Docking protein CAIR 1, CAIR 1, BIS, Bcl-2-binding protein Bis, Bcl-2-associated athanogene 3, BCL2 binding athanogene 3, BCL2 associated athanogene 3, Bcl 2 binding protein, BAG family molecular chaperone regulator 3, BAG 3 |
Ig Type | Rabbit IgG |
克隆号 | 8D844 |
交叉反应 | Human |
验证活性 | 1. Western Blot -Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, U87 whole cell lysate -All lanes: BAG3 antibody at 0.73μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 62 KDa -Observed band size: 80 KDa 2. IHC image of TMAH-00102 diluted at 1:73.75 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
应用 | ELISA, WB, IHC |
推荐剂量 | WB:1:500-1:5000; IHC:1:50-1:200. |
抗体种类 | Monoclonal |
亚细胞定位 | Nucleus. Cytoplasm. |
构建方式 | Recombinant Antibody |
纯化方式 | Affinity-chromatography |
性状 | Liquid |
缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
研究背景 | Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70. Has anti-apoptotic activity. Plays a role in the HSF1 nucleocytoplasmic transport. |
偶联 | Unconjugated |
免疫原 | A synthetic peptide: Human BAG3 |
抗原种属 | Human |
基因ID | 9531 |
Uniprot ID | |
研究领域 | Cell Biology |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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