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Anti-B2M/beta 2-Microglobulin Antibody (2F545)

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产品编号 TMAY-02181

Anti-B2M/beta 2-Microglobulin Antibody (2F545) 是一种 Rabbit 抗体,靶向 B2M/beta 2-Microglobulin。Anti-B2M/beta 2-Microglobulin Antibody (2F545) 可用于 WB,IP。

Anti-B2M/beta 2-Microglobulin Antibody (2F545)

Anti-B2M/beta 2-Microglobulin Antibody (2F545)

Rating icon 还可以
产品编号 TMAY-02181

Anti-B2M/beta 2-Microglobulin Antibody (2F545) 是一种 Rabbit 抗体,靶向 B2M/beta 2-Microglobulin。Anti-B2M/beta 2-Microglobulin Antibody (2F545) 可用于 WB,IP。

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产品介绍

生物活性
产品描述
Anti-B2M/beta 2-Microglobulin Antibody (2F545) is a Rabbit antibody targeting B2M/beta 2-Microglobulin. Anti-B2M/beta 2-Microglobulin Antibody (2F545) can be used in WB,IP.
Ig Type
Monoclonal Rabbit IgG
克隆号
2F545
交叉反应
Human
验证活性
1. Anti-B2M rabbit monoclonal antibody at 1:500 dilution.

-Lane A: Hela Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:14 kDa.

-Observed band size:14 kDa.

2. B2M was immunoprecipitated using:

-Lane A:0.5 mg HL-60 Whole Cell Lysate.

-Lane B:0.5 mg A431 Whole Cell Lysate.

-Lane C:0.5 mg Hela Whole Cell Lysate.

-Lane D:0.5 mg Raji Whole Cell Lysate

-0.5 µL anti-B2M rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-B2M rabbit monoclonal antibody, at 1:500 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 14 kDa.

-Observed band size: 14 kDa
应用
WB,IP
推荐剂量
WB: 1:500-1:1000; IP: 0.2-1 μL/mg of lysate
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Human B2M / beta-2 microglobulin (rh B2M / beta-2 microglobulin; TMPY-01686; NP_004039.1; Met 1-Met 119).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景B2M, also known as β2-Microglobulin or CDABP0092, is a component of MHC class I molecules found expression in all nucleated cells (excludes red blood cells). The major function of MHC class I moleculesis is to display fragments of proteins from within the cell to T-cells and cells containing foreign proteins will be attacked. B2M (β2-Microglobulin) is a low molecular weight protein. It was demonstrated that B2M (β2-Microglobulin) was localized in the membranes of nucleated cells and was found to be associated with HL-A antigens. B2M (β2- Microglobulin) is present in free form in various body fluids and as a subunit of histocompatibility antigens on cell surfaces lateral to the α3 chain. Unlike α3, β2 has no transmembrane region. Directly above β2 lies the α1 chain, which itself is lateral to the α2. In the absence of B2M (β2 microglobulin), very limited amounts of MHC class I (classical and non-classical) molecules can be detected on the surface. In the absence of MHC class I, CD8 T cells, a subset of T cells involved in the development of acquired immunity cannot develop. Low levels of B2M (β2 microglobulin) can indicate non-progression of HIV.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Human B2M / beta-2 microglobulin protein (TMPY-01686)
抗原种属
Human
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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