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顶部产品介绍抗原信息化学信息储存和溶解信息计算器

Anti-ATP7B Polyclonal Antibody

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货号 TMAB-00171

别名 WND/140 kDa, WND, Wilson disease associated protein, WD, WC1, tx, Toxic milk, PWD, Cu++ transporting, Cu(2+) transporting, Copper transporting ATPase 2, Copper pump 2, beta polypeptide, ATPase, ATP7B

Anti-ATP7B Polyclonal Antibody 是一种 Rabbit 抗体，靶向 ATP7B。Anti-ATP7B Polyclonal Antibody 可用于 IF,IHC-Fr,IHC-P。

Anti-ATP7B Polyclonal Antibody

Anti-ATP7B Polyclonal Antibody

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货号 TMAB-00171 别名 WND/140 kDa, WND, Wilson disease associated protein, WD, WC1, tx, Toxic milk, PWD, Cu++ transporting, Cu(2+) transporting, Copper transporting ATPase 2, Copper pump 2, beta polypeptide, ATPase, ATP7B

Anti-ATP7B Polyclonal Antibody 是一种 Rabbit 抗体，靶向 ATP7B。Anti-ATP7B Polyclonal Antibody 可用于 IF,IHC-Fr,IHC-P。

规格价格库存数量
50 μL
¥ 1,175
5日内发货
100 μL
¥ 1,960
5日内发货
200 μL
¥ 2,795
5日内发货
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产品介绍

生物活性
产品描述
Anti-ATP7B Polyclonal Antibody is a Rabbit antibody targeting ATP7B. Anti-ATP7B Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P.
别名WND/140 kDa, WND, Wilson disease associated protein, WD, WC1, tx, Toxic milk, PWD, Cu++ transporting, Cu(2+) transporting, Copper transporting ATPase 2, Copper pump 2, beta polypeptide, ATPase, ATP7B
Ig Type
IgG
反应种属
Human,Mouse,Rat (predicted:Chicken,Pig,Rabbit)
验证活性
1. Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ATP7B) Polyclonal Antibody, Unconjugated (TMAB-00171) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ATP7B) Polyclonal Antibody, Unconjugated (TMAB-00171) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ATP7B) Polyclonal Antibody, Unconjugated (TMAB-00171) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ATP7B) Polyclonal Antibody, Unconjugated (TMAB-00171) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ATP7B) Polyclonal Antibody, Unconjugated (TMAB-00171) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ATP7B) Polyclonal Antibody, Unconjugated (TMAB-00171) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ATP7B) Polyclonal Antibody, Unconjugated (TMAB-00171) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ATP7B) Polyclonal Antibody, Unconjugated (TMAB-00171) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ATP7B) Polyclonal Antibody, Unconjugated (TMAB-00171) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
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应用IFIHC-FrIHC-P
推荐剂量
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Isoform 2: Cytoplasm. WND/140 kDa: Mitochondrion.
组织特异性Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver. The cleaved form WND/140 kDa is found in liver cell lines and other tissues.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
浓度1 mg/mL
研究背景ATP7b is an important protein for copper transport and elimination of excess copper from the body. ATP7b transports metals in and out of cells using ATP. There are 3 known isoforms of the ATP7b gene; A is found in the liver, kidney, and brain, the shorter form B is found in brain tissue, and the third isoform, known as WND/140 KDA is found in mitochondria. Mutations in the ATP7b gene can cause Wilson's disease, an inherited disorder causing copper poisoning in the brain and liver.
抗原信息
免疫原
KLH conjugated synthetic peptide: human ATP7B
抗原种属
Human
基因名称
ATP7B
基因ID
540
蛋白名称
BTLA
Uniprot ID
P35670
研究领域
Vitamins / minerals,Cancer,Vitamins / Minerals
功能
Involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile.
化学信息
分子量Theoretical: 161 kDa.
储存&溶解度
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.
存储store at low temperature | store at -20°C

计算器

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