Anti-ATM Antibody (7Y845)

Name: Anti-ATM Antibody (7Y845)
Brand: TargetMol
SKU: TMAH-00088
Rating: 4.5 (1 reviews)

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产品编号 TMAH-00088

别名 telomere maintenance 1, Telomere fusion protein, TELO1, TEL1, Tefu, Serine/threonine-protein kinase ATM, Serine protein kinase ATM, OTTHUMP00000232981, MGC74674, homolog, DKFZp781A0353, ATM serine/threonine kinase, ATE, ATDC, ATD, ATC, Ataxia telangiectasia mutated homolog, Ataxia telangiectasia mutated, ATA, AT1, A-T mutated homolog, A-T mutated, AT mutated

Anti-ATM Antibody (7Y845) 是一种抗体，靶向 ATM。Anti-ATM Antibody (7Y845) 可用于 ELISA, WB, IHC, IP。

Anti-ATM Antibody (7Y845)

还可以

产品编号 TMAH-00088 别名 telomere maintenance 1, Telomere fusion protein, TELO1, TEL1, Tefu, Serine/threonine-protein kinase ATM, Serine protein kinase ATM, OTTHUMP00000232981, MGC74674, homolog, DKFZp781A0353, ATM serine/threonine kinase, ATE, ATDC, ATD, ATC, Ataxia telangiectasia mutated homolog, Ataxia telangiectasia mutated, ATA, AT1, A-T mutated homolog, A-T mutated, AT mutated

Anti-ATM Antibody (7Y845) 是一种抗体，靶向 ATM。Anti-ATM Antibody (7Y845) 可用于 ELISA, WB, IHC, IP。

规格	价格	库存	数量
50 μL	¥ 1,300	5日内发货
100 μL	¥ 2,185	5日内发货

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产品描述	Anti-ATM Antibody (7Y845) is an antibody targeting ATM. Anti-ATM Antibody (7Y845) can be used in ELISA, WB, IHC, IP.
别名	telomere maintenance 1, Telomere fusion protein, TELO1, TEL1, Tefu, Serine/threonine-protein kinase ATM, Serine protein kinase ATM, OTTHUMP00000232981, MGC74674, homolog, DKFZp781A0353, ATM serine/threonine kinase, ATE, ATDC, ATD, ATC, Ataxia telangiectasia mutated homolog, Ataxia telangiectasia mutated, ATA, AT1, A-T mutated homolog, A-T mutated, AT mutated
Ig Type	Rabbit IgG
克隆号	7Y845
交叉反应	Human
验证活性	1. Western Blot -Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, PC3 whole cell lysate, HepG2 whole cell lysate -All lanes: ATM antibody at 2.05μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 350 KDa -Observed band size: 350 KDa 2. IHC image of TMAH-00088 diluted at 1:205 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 3. IHC image of TMAH-00088 diluted at 1:205 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 4. Immunoprecipitating ATM in PC3 whole cell lysate -Lane 1: Rabbit control IgG instead of TMAH-00088 in PC3 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) -Lane 2: TMAH-00088 (3μg) + PC3 whole cell lysate (500μg) -Lane 3: PC3 whole cell lysate (20μg)
应用	ELISA WB IHC IP
推荐剂量	WB:1:500-1:5000; IHC:1:50-1:200; IP:1:200-1:1000.
抗体种类	Monoclonal
亚细胞定位	Nucleus. Cytoplasmic vesicle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
构建方式	Recombinant Antibody
纯化方式	Affinity-chromatography
性状	Liquid
缓冲液	Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
研究背景	Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates DYRK2, CHEK2, p53/TP53, FBXW7, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, UFL1, RAD9, UBQLN4 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends. Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation. Phosphorylates ATF2 which stimulates its function in DNA damage response. Phosphorylates ERCC6 which is essential for its chromatin remodeling activity at DNA double-strand breaks.