Anti-ATM Antibody (2T630)

Name: Anti-ATM Antibody (2T630)
Brand: TargetMol
SKU: TMAH-00089
Rating: 4.5 (1 reviews)

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产品编号 TMAH-00089

别名 telomere maintenance 1, Telomere fusion protein, TELO1, TEL1, Tefu, Serine/threonine-protein kinase ATM, Serine protein kinase ATM, OTTHUMP00000232981, MGC74674, homolog, DKFZp781A0353, ATM serine/threonine kinase, ATE, ATDC, ATD, ATC, Ataxia telangiectasia mutated homolog, Ataxia telangiectasia mutated, ATA, AT1, A-T mutated homolog, A-T mutated, AT mutated

Anti-ATM Antibody (2T630) 是一种抗体，靶向 ATM。Anti-ATM Antibody (2T630) 可用于 ELISA, IHC, FC。

Anti-ATM Antibody (2T630)

还可以

产品编号 TMAH-00089 别名 telomere maintenance 1, Telomere fusion protein, TELO1, TEL1, Tefu, Serine/threonine-protein kinase ATM, Serine protein kinase ATM, OTTHUMP00000232981, MGC74674, homolog, DKFZp781A0353, ATM serine/threonine kinase, ATE, ATDC, ATD, ATC, Ataxia telangiectasia mutated homolog, Ataxia telangiectasia mutated, ATA, AT1, A-T mutated homolog, A-T mutated, AT mutated

Anti-ATM Antibody (2T630) 是一种抗体，靶向 ATM。Anti-ATM Antibody (2T630) 可用于 ELISA, IHC, FC。

规格	价格	库存	数量
50 μL	¥ 1,315	5日内发货
100 μL	¥ 2,190	5日内发货

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产品描述	Anti-ATM Antibody (2T630) is an antibody targeting ATM. Anti-ATM Antibody (2T630) can be used in ELISA, IHC, FC.
别名	telomere maintenance 1, Telomere fusion protein, TELO1, TEL1, Tefu, Serine/threonine-protein kinase ATM, Serine protein kinase ATM, OTTHUMP00000232981, MGC74674, homolog, DKFZp781A0353, ATM serine/threonine kinase, ATE, ATDC, ATD, ATC, Ataxia telangiectasia mutated homolog, Ataxia telangiectasia mutated, ATA, AT1, A-T mutated homolog, A-T mutated, AT mutated
Ig Type	Rabbit IgG
克隆号	2T630
交叉反应	Human
验证活性	1. IHC image of TMAH-00089 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 2. IHC image of TMAH-00089 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 3. Overlay histogram showing Hela cells stained with TMAH-00089 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/110^6 cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/110^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.
应用	ELISA, IHC, FC
推荐剂量	IHC:1:50-1:200; FC:1:20-1:200.
抗体种类	Monoclonal
亚细胞定位	Nucleus. Cytoplasmic vesicle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
构建方式	Recombinant Antibody
纯化方式	Affinity-chromatography
性状	Liquid
缓冲液	Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
研究背景	Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates DYRK2, CHEK2, p53/TP53, FBXW7, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, UFL1, RAD9, UBQLN4 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends. Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation. Phosphorylates ATF2 which stimulates its function in DNA damage response. Phosphorylates ERCC6 which is essential for its chromatin remodeling activity at DNA double-strand breaks.