Anti-ATG18 Antibody (8B338) is a Rabbit antibody targeting ATG18. Anti-ATG18 Antibody (8B338) can be used in FCM, IF, IHC-Fr, IHC-P, WB.

IgG

8B338

Human,Mouse,Rat

1. 25 ug total protein per lane of various lysates (see on figure) probed with ATG18 monoclonal antibody, unconjugated (TMAB-02915) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The U-87MG (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-ATG18 antibody (TMAB-02915, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

FCM=1:50-100; IF=1:100-200; IHC-Fr=1:100-200; IHC-P=1:100-200; WB=1:500-2000

Monoclonal

A synthesized peptide: human WIPI 1

Human

WIPI1

WD repeat domain phosphoinositide-interacting protein 1

Plays an important role in autophagy and in particular starvation- and calcium-mediated autophagy, as well as in mitophagy. Functions upstream of the ATG12-ATG5-ATG16L1 complex and LC3, and downstream of the ULK1 and PI3-kinase complexes. Involved in xenophagy of Staphylococcus aureus. Invading S.aureus cells become entrapped in autophagosome-like WIPI1 positive vesicles targeted for lysosomal degradation. Plays also a distinct role in controlling the transcription of melanogenic enzymes and melanosome maturation, a process that is distinct from starvation-induced autophagy. May also regulate the trafficking of proteins involved in the mannose-6-phosphate receptor (MPR) recycling pathway.