Anti-ATAD2 Antibody (4D366) is a Rabbit antibody targeting ATAD2. Anti-ATAD2 Antibody (4D366) can be used in WB,FCM.

IgG

4D366

Human

1. 25 μg total protein per lane of various lysates (see on figure) probed with ATAD2 monoclonal antibody, unconjugated (TMAB-02887) at 1: 2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The MCF-7 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Rabbit Anti-ATAD2 antibody (TMAB-02887): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

WB: 1:500-2000; FCM: 1μg/Test

Monoclonal

A synthesized peptide: human ATAD2

Human

ATAD2

ATPase family AAA domain-containing protein 2

May be a transcriptional coactivator of the nuclear receptor ESR1 required to induce the expression of a subset of estradiol target genes, such as CCND1, MYC and E2F1. May play a role in the recruitment or occupancy of CREBBP at some ESR1 target gene promoters. May be required for histone hyperacetylation. Involved in the estrogen-induced cell proliferation and cell cycle progression of breast cancer cells.