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Anti-ASGR1 Antibody (8E417)
还可以产品编号 TMAY-01152
Anti-ASGR1 Antibody (8E417) 是一种 Rabbit 抗体，靶向 ASGR1。Anti-ASGR1 Antibody (8E417) 可用于 WB,ELISA(Cap),IP。
Anti-ASGR1 Antibody (8E417)
Anti-ASGR1 Antibody (8E417)
还可以产品编号 TMAY-01152
Anti-ASGR1 Antibody (8E417) 是一种 Rabbit 抗体，靶向 ASGR1。Anti-ASGR1 Antibody (8E417) 可用于 WB,ELISA(Cap),IP。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-ASGR1 Antibody (8E417) is a Rabbit antibody targeting ASGR1. Anti-ASGR1 Antibody (8E417) can be used in WB,ELISA(Cap),IP. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 8E417 |
| 交叉反应 | Human |
| 验证活性 | 1. Anti-ASGR1 rabbit monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:33 kDa. -Observed band size:43 kDa. 2. ASGR1 was immunoprecipitated using: -Lane A:0.5 mg HepG2 Whole Cell Lysate. -4 µL anti-ASGR1 rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G. -Primary antibody: -Anti-ASGR1 rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size: 33 kDa. -Observed band size:43 kDa |
| 应用 | WB,ELISA(Cap),IP |
| 推荐剂量 | WB: 1:500-1:2000; IP: 1-4 μL/mg of lysate; ELISA(Cap): 1:250-1:2000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human ASGPR1 (rh ASGPR1 ; TMPY-02702; P07306; Gln62-Leu291). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | The asialoglycoprotein receptor (ASGPR), an endocytotic cell surface receptor expressed by hepatocytes, is triggered by triantennary binding to galactose residues of macromolecules such as asialoorosomucoid (ASOR). ASGPR belongs to the long-form subfamily of the C-type/Ca2+ dependent lectin family. It is a complex of two noncovalently-linked and highly homologous subunits, a major 42 kDa glycoprotein ASGPR1(MHL-1) and a minor 51 kDa glycoprotein ASGR2 (MHL-2). ASGPR1 is synthesized as a type II transmembrane protein that contains a cytosolic N-terminal domain, a single transmembrane segment, and an extracellular domain which contains two important structural regions. The first is a stalk domain that contributes to noncovalent oligomerization, and the second is a Ca2+-dependent carbohydrate binding domain at the very C-terminus that is unusually stabilized by three ions. The research regarded that ASGPR1 could be targeted for anti- hepatitis B virus (HBV) drug development. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human ASGPR1 protein (TMPY-02702) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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