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Anti-ARF6 Antibody (4X904) 是一种 Rabbit 抗体,靶向 ARF6。Anti-ARF6 Antibody (4X904) 可用于 WB,ICC/IF。
Anti-ARF6 Antibody (4X904) 是一种 Rabbit 抗体,靶向 ARF6。Anti-ARF6 Antibody (4X904) 可用于 WB,ICC/IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,485 | 5日内发货 | |
100 μL | ¥ 2,490 | 5日内发货 |
产品描述 | Anti-ARF6 Antibody (4X904) is a Rabbit antibody targeting ARF6. Anti-ARF6 Antibody (4X904) can be used in WB,ICC/IF. |
别名 | Small GTPase, Small GTP binding protein, DKFZp564M0264, ARF6_HUMAN, ARF 6, ADP-ribosylation factor 6, ADP ribosylation factor protein 6, ADP ribosylation factor 6 |
Ig Type | IgG |
克隆号 | 4X904 |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of ARF6 on human liver lysates using anti-ARF6 antibody at 1/1,000 dilution. 2. ICC staining ARF6 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining ARF6 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining ARF6 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
应用 | |
推荐剂量 | WB: 1:1000-2000; ICC/IF: 1:100-500 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | The ADP-ribosylation factor (ARF) protein family are structurally and functionally conserved members of the Ras superfamily of regulatory GTP-binding proteins. ARFs influence vesicle trafficking and signal transduction in eukaryotic cells. ARF-dependent regulatory mechanisms include the coordination of spectrin interactions with golgi membranes and the association of Actin to the Golgi via rho family-dependent G-protein localization (Rac, CDC42) and WASP/Arp2/3 complexes. Additionally, ARFs play a central role in maintenance of organelle integrity, assembly of coat proteins and activation of phospholipase D. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6); members of each class share a common gene organization. The human ARF6 gene contains five exons and four introns, and encodes a 175 amino acid protein. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 20 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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