Anti-Aquaporin-7/AQP7 Polyclonal Antibody is a Rabbit antibody targeting Aquaporin-7/AQP7. Anti-Aquaporin-7/AQP7 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow)

1. Blank control: A549. Primary Antibody (green line): Rabbit Anti-AQP7 antibody (TMAB-00141)
Dilution: 3 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 3 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Tissue/cell: Mouse embryos; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-AQP7 Polyclonal Antibody, Unconjugated (TMAB-00141) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Black line: Positive blank control (A549); Negative blank control (HUVEC)
Green line: Primary Antibody (Rabbit Anti-AQP7 antibody (TMAB-00141))
Orange line: Isotype Control Antibody (Rabbit IgG).
Blue line: Secondary Antibody (Goat anti-rabbit IgG-AF488)
A549 (Positive) and HUVEc (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with AQP7 Antibody (TMAB-00141) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody (blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
4. Sample:
Lane 1: Adipose (Mouse) Lysate at 40 μg
Lane 2: Kidney (Mouse) Lysate at 40 μg
Lane 3: Testis (Mouse) Lysate at 40 μg
Lane 4: Spleen (Mouse) Lysate at 40 μg
Primary: Anti-AQP7 (TMAB-00141) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37/18 kDa
Observed band size: 40 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 2ug/test

Polyclonal

KLH conjugated synthetic peptide: human AQP7

Human

AQP7

Aquaporin-7

Channels,Cancer

Forms a channel for water and glycerol (By similarity).